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            Seaweeds, particularly the red seaweed Asparagopsis taxiformis, produce and sequester bromomethanes, which are known for mitigating methane emissions in ruminants when used as a feed supplement. Bromomethane synthesis requires hydrogen peroxide (H2O2). We developed a staining assay utilizing 3,3′-diaminobenzidine (DAB) for identifying H2O2 in three groups of seaweeds (red, brown, and green), including intensely pigmented species. Our findings indicate the previously identified "gland cell" in Asparagopsis taxiformis, responsible for bromoform synthesis and retention, is a specialized large organelle rich in H2O2. Our study introduces an effective survey tool to identify promising seaweed species abundant in bromoform from diverse marine habitats.more » « less
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